Protein Solutions
Calorimetry, Research and Consulting


Protein Solutions provides a range of scientific services such as assistance in experimental research, theory-based interpretation of data, as well as "know how" and consulting in protein/peptide stability and thermodynamics.

Upcoming Events

July 18, 2012

World Drug Target/Deliver Online Symposium Series

Past Events

2011

Lecture presentation at GE Healthcare meeting on label-free technology in Copenhagen University, Denmark

Lecture presentation at TA Instruments workshop and seminar at Roskilde University, Denmark

Experimental assistance and project-based research

All experimental research is conducted on customer's site, using the provided equipment and supplies. Characterization of proteins, peptides, liquid formulations and so on is performed using Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry (DSC), Circular Dichroism (CD), spectrophotometry and Dynamic Light Scattering (DLS).

The amount of experimental work, required for completion of the project depends on your needs. A simple assesment of protein thermal stability at defined solution conditions takes one day, whereas mapping thermal stability through the range of pH values or salt concentrations will take about 2 weeks. It is possible to arrange both short trial experiments as well as larger investigations directed towards overall characterization of macromolecular systems.

It is a good idea to supplement the thermal stability studies with the structural characterization, as protein biological activity depends largely on its structure. This is done with the help of far and near UV CD, as this quick and simple method provides an overall assessment of both secondary and tertiary structure.

In many cases understanding of protein physico-chemical properties is further enriched as more methods are applied to clarify the details of the molecular changes induced by temperature, co-solvents, ligands, and so on, leading to in-depth understanding of protein stability profile. This knowledge can be applied for defining suitable purification conditions, successful stabilization strategies and/or assessment of drug interaction with possible stabilizers, inhibitors, etc.